Host: The Japan Radiation Research Society
Co-host: Asian Association for Radiation Research
Because of the accomplishment of the human genome project and development of molecular biological techniques including microarray analysis, it is now possible to investigate the common structure of radiation–responsive genes and genes involved in radiation sensitivity of mammalian cells. To reveal gene expression network after exposure to ionizing radiation, it is important to analyse and describe the common structure of the transcriptional regulatory region of ionizing radiation–resonsure genes promoter in the proximity of the mRNA start site. Jeong Sun Seo, Vivian G Cheung, Sally A Amundson, et al. have identified human genes responsive to gamma-rays by a microarray analysis. Here, we attempted to reveal a common structural character of the core promoter of 217 kinds of ionizing radiation–responsive genes by examining the frequency of TATA–box, initiator, GC–box, CCAAT–box element. We used the Match software with investigated the frequency of each factor under the same conditions (the optimum cutoff value and the search range). The frequency of TATA–box, initiator, GC–box and CCAAT–box were 35.50%, 94.90%, 88.94% and 44.70%, respectively, in the 217 genes responsive to gamma–rays. By comparing with promoters of randomly selected genes, TATA–box was ascertained that there isn’t a significant difference between two groups. However, the frequency of the initiator was 10% higher than that in usual genes. Frequencies of GC–box and CCAAT–box were 8% and 20% lower, respectively.
