Establishment of new systems to analyze the effect of DNA double strand break(DSB)

Abstract

Irradiation causes various DNA damages, directly or indirectly. One of the important damages is the DSB, which could be followed by huge genomic alteration such as DNA deletion or genomic rearrangement. Thus the repair of DSB is significant process to maintain genomic information. At the first step of the repair, it could be crucial to change chromatin status for increasing the accessibility of repair enzymes. In this context, the modification of histones at the DSB site is to be elucidated. We are establishing a new system to harvest local chromatins in the mouse genome. Using this system, the kinetic change of chromatines at the DSB site could be analysed.