Host: The Japan Radiation Research Society
Biological dosimetry is very important as the first step in the medical treatment of the irradiated persons. Since it has been indicated that the frequency of chromosome aberrations correlates with the radiation dose, it is considered to be the most reliable, specific and sensitive biomarker for dose estimation. In general, the peripheral lymphocytes are used for the analysis of the dicentric assay in the exposed persons. To obtain the accurate frequency of the dicentric chromosome in dose estimation, it may be significant to recognize clearly the primary constriction as the landmark of centromere on chromosome. It has also been suggested that the degree of chromosome condensation may have significant effects on the analysis of dicentric chromosome. In order to investigate the effects of Colcemid treatment on chromosome condensation, we compared the relative length of chromosome 2 in the lymphocytes which were treated with the concentrations of 0.01, 0.03, 0.05 and 0.1ug/ml for 2, 24 and 48hrs. It is very clear that the elongated chromosomes were obtained from the lymphocytes treated with 0.01ug/ml for 2 hrs. However, there was no significant differences in the relative length of chromosome 2 between the lymphocytes treated with 0.03, 0.05 and 0.1ug/ml for 24 and 48hrs.
