Host: The Japan Radiation Research Society
Biological dosimetry is considered to be very important as the first step in the medical treatment for the persons exposed to radiation. Since the frequency of dicentric chromosome correlates with the radiation dose, it is considered to be the most reliable, specific and sensitive biomarker for dose estimation. In the present study, a modified C-band technique was developed in order to analyze more accurately dicentric, tricentric and ring chromosomes in irradiated human peripheral lymphocytes. Instead of the original method due to the treatment with barium hydroxide Ba(OH)2, C-bands were obtained from the modification of heat treatment in formamide and following DAPI staining. This method was applied to the analysis of dicentric chromosome in irradiated human lymphocytes to examine its availability. The frequency of dicentric chromosome was almost equal between conventional Giemsa staining and the modified C-band technique. Although the analysis by Giemsa staining is comparatively difficult to recognize the centromere on the elongated chromosomes, the over-condensed chromosomes, a fragment and an acentric ring, our modified C-band method may be easier to identify the centromere on such chromosomes than Giemsa stain only. Thus, the modified C-band method may give more information about the location of centromere. Therefore, this method may be available and more useful for biological dose estimation due to the analysis of dicentric chromosome in human lymphocytes exposed to the radiation.
