Abstract
Radio-protective effect of AA-2G (L-ascorbic acid 2-glicoside ) was evaluated in apoptosis induction of mouse spleen after total body 5Gy irradiation. We also investigated the effect of AA-2G for the anti-oxidative enzymes activation (catalase and superoxide dismutase) in plasma and splenocytes following irradiation. The ascorbic acid concentration in mouse plasma increased and maintained a high level during 30-90 min after oral administration of AA-2G. After 1 hour of AA-2G administration at the concentration of 25mg/body, the concentration of the ascorbic acid in plasma was increased 4-10 times higher than the control. Mice were irradiated in plastic tubes by gamma-ray at 5 Gy. The splenocytes were isolated 1-24 hours after total body irradiation and the apoptosis was measured with Elisa kit (Roche). The activation of the anti-oxidative enzymes in plasma and splenocytes was measured by catalase or SOD assay kit (Calbiochem). The treatment of AA-2G reduced the apoptosis induction of mouse splenocytes and increased the activation of catalase in splenocytes following irradiation.
