Abstract
[Purpose]
The thermo-enhancement effects of Parthenolide (PTL), a principal ingredient of herb which target on NF-kappaB, transcript factor, were investigated in human lung adenocarcinoma A549 cells with wtp53, show the low thermosensitivity. The expression of heat induced hsp70 proteins and p53 proteins, takes part in apoptosis induction, were also examined. The thermo- enhancement effects of PTL through the signaling pathway of NF-kappaB were analyzed.
[Materials & Methods]
A549 cells, human lung adenocarcinoma, were used in this study. Cytotoxicity or thermosensitivity to PTL, hyperthermia or the combination therapy in A549 cells were estimated by colony formation assay. The kinetics induction of hsp70 and p53 proteins after treatment with PTL, hyperthermia or the combination was analyzed by western blot assay. Induction of apoptosis was analyzed using fluorescent dye Hoechst33342.
[Results]
Combination therapy with PTL and hyperthermia in A549 cells showed thermo-enhancement effects. After treatment with hyperthermia alone, the accumulated amount of hsp70 increased markedly, but with PTL alone did not increase. Both hsp70 and p53 proteins were not induced significantly after combined treatment with PTL and hyperthermia. Apoptosis after treatment with PTL showed remarkable incidences, while apoptosis after combined treatment with PTL and hyperthermia showed significant incidences.
[Summary]
Thermosensitivity of human lung adenocarcinoma A549 cells was enhanced synergistically in combination therapy with PTL and hyperthermia. Heat-induced apoptosis after combined treatment with PTL and hyperthermia showed increase significantly. The thermo-enhancement effects of PTL were not inhibition of heat-induced hsp70, but due to induce apoptosis by blocking the activation of NF-kappaB.
