Abstract
We have reported that low-dose irradiation enhanced antioxidant function and alleviated the ischemia-reperfusion injury in mouse. It was also reported that organ transplantation induced ischemia-reperfusion injury. In the present study, we examined the changes of the antioxidant substances in graft mouse liver after low-dose X-irradiation. BALB/c mouse liver was irradiated by sham, 0.25, 0.5, 1.0, or 5.0 Gy and stored for 4, 8, 24, or 48 hours in the preservative (Via Span) solution containing glutathione or saline solution. Results show that the activities of SOD and catalase were significantly increased for 24 hours soakage in saline solution after 0.25 or 0.5 Gy irradiation or in preservative solution after 1.0 Gy irradiation. The glutathione content was significantly decreased for 24 hours soakage in saline solution or preservative solution after every irradiation. On the other hand, after 0.5 Gy irradiation, the activities of SOD (4 or 24 hours) and catalase (8 hours) were significantly increased by soakage in saline solution. And the glutathione contents were significantly decreased for over 4 hours soakage in saline solution or preservative solution. Moreover the lipid peroxide level was significantly decreased for 8 hours soakage in saline solution. These findings suggested that low-dose irradiation significantly activates antioxidant function of mouse liver in saline solution or preservative solution. On the other hand, the preservative solution contains glutathione. And glutathione was made synthetically from ATP, which was decreased during ischemia. Therefore the glutathione content was significantly decreased in these conditions of irradiation and preservation.
