Abstract
We are accumulating physiological information after exposure organisms to environmental stress using OMICS (Genomics, Proteomics, and Metabolomics) technology. Environmental stress includes radiations, high temperature, low temperature, high pressure, chemicals and so on. As the model organisms, we selected yeast, rice, medaka, rat, and human cell lines. In this workshop, we will focus on the stress response of yeast cells caused by radiation comparing with other stress response. Further, we also talk the possibility of medaka, microorganisms and rice as the target of OMICS technology.
the irradiations, heavy-ion (C) (6.0,12.0,25.0 Gy, National Institute of Radiological Sciences), thermal neutron (4.0, 6.9, 20.8 Gy, Kyoto University), fast neutron (3.3, 9.7, 21.4 Gy, Hiroshima University), gamma ray (3.3, 5.8, 17 Gy, Hiroshima University). The yeast Saccharomyces cerevisiae, strain S288C was used as the indicator strain for DNA microarray analysis. Under irradiation conditions, yeast cells were kept in a resting state at low temperature. Cell suspensions in cuvets or culture flasks were irradiated. After irradiation, cells were resuspended in YPD medium (50 ml) and cultured for 40 min. We analyzed mRNA expression profiles from the cells exposed to radiation with approximately 20 Gy, as we could confirm biological effect to yeast cells. In 6000 yeast genes, we could detected induced genes with the number of 92 genes (heavy-ion), 222 genes (thermal neutron), 37 genes (fast neutron), and 220 genes (gamma ray) and repressed genes with that of 38, 131, 73, 151 genes. We could also selected specifically induced or repressed genes by each irradiation with 3, 162, 31, 231 genes and 12, 88, 34, 321genes. We will characterize these genes on site.
