Abstract
HiCEP (High-coverage expression profiling) is a novel comprehensive analysis method which is based on DNA finger printing and PCR amplification. It enables to detect any altered gene expression among 60-70% of all the actually transcribed genes in any eukaryotic cells and tissues. We discovered interesting response to X-rays at very low dose by using HiCEP. The strategy successfully detected the very small alteration of the gene expression, thus promising as a useful tool to develop risk assessment for environmental toxic reagents. Another advantage of HiCEP over other prevailing methods is that it can be applied to most animals and plants on the earth regardless of the availability of their genome information, which will promote us to explore many useful biomarkers.
