The cell proliferation change by irradiation in developing medaka brain

Abstract

In a preveous study, we describe a novel method of rapid and quantitative evaluation of the degree of radiation-induced apoptosis in the developing brain of medaka. Twenty to twenty four hours after the irradiation, living embryos were stained with a vital dye, acridine orange, for 2 h, and whole mount brains were examined under an epifluorescence microscope. The numbers of AO stained rosette shaped nuclear clusters and AO stained single nuclei increased in a dose dependent manner in the optic tectum.In this study, to examine the proliferation change, we carried out an immunohistochemical analysis using an antibody against a marker of proliferation, phospho histone H3.