Evaluation of H2AX focus observed in the Ku80 deficient CHO mutant (xrs5) cells following gamma ray irradiation

Yuko KINASHI; Genro KASHINO; Yong LIU; Minoru SUZUKI; Shin-ichiro MASUNAGA; Sentaro TAKAHASHI; Koji ONO; Ryuichi OKAYASU

doi:10.11513/jrrsabst.2009.0_100_2

The 52nd Annual Meeting of the Japan Radiation Research Society

Session ID : OD-4

DOI https://doi.org/10.11513/jrrsabst.2009.0_100_2

Conference information

Host: The Japan Radiation Research Society, Chairman of the 52nd Annual Meeting, Toshiteru Okubo (Radiation Effects Research Foundation)

DNA damage/repair 2

Evaluation of H2AX focus observed in the Ku80 deficient CHO mutant (xrs5) cells following gamma ray irradiation

*Yuko KINASHI, Genro KASHINO, Yong LIU, Minoru SUZUKI, Shin-ichiro MASUNAGA, Sentaro TAKAHASHI, Koji ONO, Ryuichi OKAYASU

Author information

Keywords: H2AX, xrs5, gamma ray

CONFERENCE PROCEEDINGS FREE ACCESS

Details

Abstract

Xrs5(Ku80 deficient CHO mutant cells)are well-known for the DNA double strand breaks (DBSs) repair deficiency. The amount of DNA-DSBs following gamma irradiation in xrs5 cells was evaluated by the phosphorylated H2AX and 53BP1 foci using the immunofluorescence intencity. After the 72 hours irradiation, the number of H2AX foci per xrs5 or CHO-K1 cells was 35 and 7 respectively. Our results suggested that the DNA-DSBs in xrs5 cells were not repaired 72 hours after irradiation.

Corresponding author

Register with J-STAGE for free!