Abstract
[Purpose]
Reactive oxygen species (ROS) induced by ultraviolet rays (UVR) in human skin causes extra- and intracellular oxidative stresses. It is suggested that ROS induces photo-aging phenomenon such as activation of matrix metalloproteinases and melanogenesis, and photo-carcinogenesis such as melanoma. As an UVR response in the skin, melanogenesis in melanocyte is well known. This response needs the release of cytokines such as endothelin and alpha-MSH from keratinocyte. These cytokines stimulate melanocyte and promote melanogenesis. However, the relation between UV-induced ROS and cytokines is not clear. Aim of this study is to understand the relationship of ROS and cytokines in melanogenesis, using melanoma cell, melanocyte and keratinocyte.
[Methods]
Murine B16 melanoma cells were treated with H2O2 and UV. Then, we measured the melanin content and cellular tyrosinase activity.
[Results and discussion]
In B16 cells treated with 30 microM of H2O2, the cellular tyrosinase activity increased to 2.51 times higher than that of control 24 hours after treatment, and the melanin contents increased to 1.26 times. This shows that melanogenesis is induced by oxidative stress in murine B16 melanoma cell. By this report, we show influence of UV-induced ROS on melanogensis.
