Abstract
Spectra of DNA damage (strand breakage, base lesion, damage distribution, etc.) induced by ionizing radiation are considered to be different among radiation qualities. Although investigators are obtaining information about the difference, it is difficult to conclude overall nature of DNA damage by means of only a method for detecting a DNA damage. So, it is expected that we can have new knowledge by comparing data obtained by two or more individual methodologies.
So far, we have obtained some DNA damage information of 60Co gamma-rays, etc., by comparing data from analytical methods. For example, in yields for single strand break on dry DNA sample induced by 60Co gamma-rays, we found that the yield obtained by using phosphodiesterase I [1], by which calculating an absolute strand break yield, is 1.3 - fold larger than that obtained by super coiled plasmid method (utilizing its conformational changes by a strand breakage). This result implies that it is necessary for us to conclude nature of the damage under sufficient discussion about nature of a methodology. Particularly, it is quite important for us to review and reconsider the methodology, particularly when investigating, e.g., chemical and spatial existence of clustered damage.
In this presentation, we will discuss an approach to obtain beneficial and ungarbled DNA damage information for contributing connection of radiation chemistry with radiation biology.
[1] Akamatsu, K., Anal. Biochem. 362 (2007) 229-235.
