The Japan Radiation Research Society Annual Meeting Abstracts
The 54th Annual Meeting of The Japan Radiation Research Society
Session ID : OG-1-1
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(6-4) photolyase and UVB sensitivity of rice
*Mika TERANISHIYuko TAKAHASHIIkuko MUNAMURAJun HIDEMA
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Keywords: (6-4)photolyase, UVB, rice
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Abstract
Ultraviolet-B (UVB) radiation induces photodamage in DNA, including the formation of cyclobutane pyrimidine dimers (CPDs) and (6-4) pyrimidine-pyrimidone photodimers [(6-4) photoproducts]. Two major repair mechanisms for such DNA damage are excision repair and photoreactivation. In photoreactivation, the enzyme photolyase mediates the repair of damaged DNA by binding to CPDs or (6-4) photoproducts in a lesion-specific manner. In plants, the CPD photolyase is a crucial factor for determining UVB sensitivity. In rice, however, the relative contribution of (6-4) photolyase to UVB resistance is unclear. We measured the activity of (6-4) photolyase and the UVB sensitivity of three rice cultivars. There was no correlation between (6-4) photolyase activity and UVB sensitivity. Furthermore, we investigated the UVB sensitivity of (6-4) photolyase-deficient mutant lines induced by rice retrotransposon Tos17. No difference was found between wild-type rice and the mutant. The Arabidopsis mutant defective in (6-4) photolyase was more sensitive to UVB than the wild-type. These results suggested that the activity of (6-4) photolyase was not significantly affected the UVB sensitivity of rice.
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