High Speed Light Sheet Microscope with Millisecond Control of Focus and Sheet Light

Abstract

Light sheet microscopes are microscopes that can capture images with low noise by illuminating only the focal plane with sheet light. In the conventional 3D measurement of light sheet microscopes, the observation object or the objective lens is moved for measurement, but the slow movement of the stage makes it difficult to control at high speed. In this study, we proposed a high-speed light-sheet microscope that uses TAG lenses and galvanometer mirrors to focus and control the sheet light in milliseconds. From the experiments using a developed prototype system, we were able to measure 3D information consisting of 10 layers of images in 10 ms, and confirmed the high speed of 100 volume/s.