Abstract
In the biotechnology field, it is very difficult to manipulate microscopic material through human handling. There is a great need for a new experiment system that is easy to operate. In this paper, we show a three dimensional calibration technique for reducing positional errors of a glass pipette tip, attached to a micro-manipulator. We then propose a method of observing the manipulator tip with sub-micron order accuracy using a newly developed lighting method with an optical fiber. We also take into account the influence of the positional error of z-axis introduced by the microscopic optical system.
