Investigation of the Effects of Atmospheric Low Temperature Plasma on Lung Adenocarcinoma Cells and Optimization of Treatment Conditions

Abstract

　Lung cancer is the leading cause of cancer-related deaths in Japan, with high morbidity rates among younger individuals and women. Treatment options primarily include surgical intervention, drug therapy, and chemotherapy, all of which pose significant psychological and physical burdens and carry high risks. In this study, we investigated a novel treatment approach using atmospheric pressure low temperature plasma (ALTP) and its effects on lung adenocarcinoma cells. Although previous research suggested that APLTP could suppress tumor growth and inhibit cell proliferation in a colorectal cancer model in rats, several issues remained, including the determination of optimal irradiation conditions and elucidation of the underlying inhibitory mechanisms. In this paper, we examined the optimal irradiation time for APLTP treatment on lung adenocarcinoma cell cultures by assessing cell motility and analyzing gene expression profiles.