Abstract
A prototype module of plasma separator, which consists of an improved cellulose acetate hollow fiber (Plasmaflo PF-02), replacing the old module of Plasmaflo (PF-0I), has been developed.
Membrane plasmapheresis with Plasmaflo (PF-02) for collection of 500ml of filtrated plasma was carried out in 7 healthy mongrel dogs (I2-I8kg) under conventional blood access and partial extracorporeal circulation, corresponding to ordinary hemodialysis at a flow rate of 50-80ml/min. Acid citrate dextrose solution (ACE) was infused, as anticoaglant, at 1/10 th of the rate of extracorporeal blood flow into the outflow blood line from the dog as close to the animal as possible and calcium gluconate solution (Calcicol) was infused at 1/I0th of the rate of ACD infusion, i.e., regional citration was used for anticoagulation. 500ml of filtrated plasma was obtained within 30 minutes. All the 7 dogs tolerated the procedure well. Hematological and biochemical parameters, except hypoproteinemia and mild transient acidosis, remained within normal limits. Biochemical components of filtrated plasma were almost sane as those of the plasma obtained by centrifugal procedure.
These experimental results indicated that this membrane plasmapheresis for collection of plasma is safe for the donor and has a great significance because of the increasing demand for larger amount of plasma in the mordern medical field.
