抄録
We previously reported that with scanning electron microscopy (SEM), the quantity and distribution in adherence of WBC and platelet onto the membrane surface of dialyser differs according to the membrane materials, sterilization methods and the design of dialyser. We also recognized the absence of correlation between WBC adhesion and hemodialysis leucopenia.
In the present study, we evaluated the adhesion of these blood components onto the membrane surface of hemofielter and activated charcoal, and also the effects of anticoagulation (heparin or prostaglandin D2).
Furthermore, we developed the new evaluating method of blood adhesion using microdensitometry.
Even if the same kind of membrane is utilized, adherence onto the hemofilter membrane was less than that onto the dialyser. Estimated with microdensitometry patterns, the distribution of WBC adhesion onto the cellulose membrane was altered with the application of PGD2 as an anticoagulant.
Platelet and WBC adhered mainly to the sutures of charcoal surface and there were no differences in adhesion between polyalcohol and polyHEMA coated ones. The adhesion was reduced with the increase of heparin dosage or the use of PGD2.
These results indicate that WBC and platelet adhesion to the surface of artificial kidney differs according to materials, modes of treatment and the anticoagulation. The SEM and microdensitometry analysis were shown to be excellent evaluating methods for blood compatibility of artificial kidney.
