Abstract
The present experiment was performed to establish the basic design of metabolic support system for a hybrid artificial liver utilizing hepatocytes. As the concept, an extracorporeal hemoperfus ion will be employed for the support system. The following points were examined that which is effective PAN and PMMA as the semipermeable membrane, which is better for hepatocytes perfusate Hank's salt solution and Waymouth's medium in point of the maintenance of hepatocyte function, what kind of perfusion system is suitable for the support system and whether an intermittent ultrafiltration system is useful for the metabolic effects, in in-vitro perfusion experiments. The results were obtained that the same range of metabolic rates were observed in both membranes, Hank's solution was not suitable for hepatocyte perfusate and that the intermittent ultrafiltration were useful for the perfusion system.
