急性肝不全時のdetoxification systemとしてのimmobilized enzymeの応用 -in vitroの検討-

田畑 陽一郎; 小高 通夫; 平沢 博之; 小林 弘忠; 林 春幸; 菅井 桂雄; 大竹 喜雄; 織田 成人; 佐藤 博; Thomas M. S. CHANG

doi:10.11392/jsao1972.11.485

APPLICATION OF IMMOBILIZED ENZYME TO THE TREATMENT OF ACUTE LIVER FAILURE AS A DETOXIFICATION SYSTEM

-in vitro study-

Yoichiro TABATA, Michio ODAKA, Hiroyuki HIRASAWA, Hirotada KOBAYASHI, Haruyuki HAYASHI, Takao SUGAI, Yoshio OHTAKE, Shigeto ODA, Hiroshi SATOH, Thomas M. S. CHANG

Author information

Yoichiro TABATA
Department of Surgery (II), Chiba University School of Medicine
Michio ODAKA
Department of Surgery (II), Chiba University School of Medicine
Hiroyuki HIRASAWA
Department of Surgery (II), Chiba University School of Medicine
Hirotada KOBAYASHI
Department of Surgery (II), Chiba University School of Medicine
Haruyuki HAYASHI
Department of Surgery (II), Chiba University School of Medicine
Takao SUGAI
Department of Surgery (II), Chiba University School of Medicine
Yoshio OHTAKE
Department of Surgery (II), Chiba University School of Medicine
Shigeto ODA
Department of Surgery (II), Chiba University School of Medicine
Hiroshi SATOH
Department of Surgery (II), Chiba University School of Medicine
Thomas M. S. CHANG
The Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University

Keywords: immobilized enzyme, immobilized UDP-glucuronyl transferase, artificial cells, liver failure, detoxification, liver support system

JOURNAL FREE ACCESS

1982 Volume 11 Issue 2 Pages 485-488

DOI https://doi.org/10.11392/jsao1972.11.485

Details

Abstract

The in-vitro removal of p-nitrophenol by means of perfusion over the immobilized UDP-glucuronyl transferase within artificial cells was studied. The assayed activities of the immobilized UDP-glucuronyl transferase were about a half of the value of the same amount of free enzyme.
The storage stabilities of immobilized enzyme and free enzyme werstudied under the temperature of 4°C, 22°C and 37°C, respectively. The results showed that immobilized enzyme was more stable than the free enzyme.
Further studies were conducted to determine the stability, kinetics of this system. The results obtained suggest that immobilized UDP-glucuronyl transferase is efficient in conjugating p-nitrophenol in vitro.

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