Abstract
One of the causes of infection with hepatitis B (HB) virus in blood or plasma transfusion. The most effective method to prevent such infection is to transfuse Dane particle negative blood or plasma.
Now, we have been looking into the possibility of eliminating Dane particle through HBs-antibody treated filter.
This paper presents the findings of the basic experiments on binding form of HBs-antibody. Immobilized HBs-antibody was prepared by coupling antihuman HBs-antibody with activated Sepharose 4B and digested by pepsin in 37°C×16 hours incubation. The fragments such as Fab' and Fc' were qualified by Ouchterlony method and measured by Gelfiltration method. The binding form was guessed from releasing pattern of fragments into experimental solution.
We obtained the some hypothesis that the ratio of formal binding and reversed Y binding was approximately 2:1, but the horizontal Y binding could not be calculated.
