Adsorptive removal of solutes from uremic plasma by hemofilter membranes

Abstract

Beta-2-microglobulin (β₂MG) was not found in the filtrate during the hemofiltration treatment using PMMA membrane hemofilter. But the plasma level of β₂MG fell effectively. This ridiculous phenomenon implies the possible adsorption of the solute by the membrane surface. We planed to reveale the adsorption affinity between the solutes in uremic plasma and the membranes, PMMA, PAN, and cuprophane. In vitro incubation study using uremic plasma and small-tipped membrane materials showed the strong affinity of PMMA with β₂MG and glucagon, and of PAN with lysozyme. Cuprophane had only weak affinity with some solutes. The adsorption experiment in closed uremic plasma circuration system using clinical modules resulted the resembling affinity properties. Definite association between in vitro and in vivo studies was not always observed. Reductions of the plasma levels of glucagon, β₂MG, ribonuclease, lysozyme were recognized by PMMA in vivo, glucagon and lyso zyme by PAN also.