Abstract
We previously clarified with the scanning electron microscopic technique that the adhesion of blood cell components to the surface of artificial kidney differed according to materials, anticoagulants and modes of treatment. In the present study, we estimated the blood cell adherence to the surface of four kinds of dialysis membrane (cellulose acetate, saponified cellulose ester, cellulose di-acetate and ethylene vinyl alcohol) and three kinds of membrane plasma separater (polymer alloy, cellulose di-acetate and PMMA). We also evaluated the effects of synthetic anti-coagulants (MD-805 and FUT-175) and blood flow rate on that. The white blood cell adhesion to all four kinds of dialysis membrane was dominant in the arterial side of hollow fiber and same distributed pattern was noted after the dialysis with either MD-805 or FUT-175. High blood flow rate or hemofiltration therapy reduced the number of adhered blood cells. Differing from the adhered pattern to dialysis membrane, considerable number of red blood cell and platelet adhered to the venous side of membrane surface in plasma separater. These results indicate that evaluation of blood cell adherence to the membrane surface with scanning electron microscopy is useful method to estimate the effects of membrane materials, anticoagulants and modes of therapy on the blood membrane surface interaction.
