Abstract
Hemolytic plasma acts on blood asa a coagulation activator. By gel filtration fractionation on Sephadex G-15, hemolytic plasma is separated into (1) a tissue thromboplastin-like substance containing proteins and (2) Redox potentialassociated ADP, AMP and ATP of the molecular weight of approximately 500 not containing proteins. The role of ADP in platelet agglutination was investigated using an aggregometer. Platelets distinctly showed agglutination in the presence of hemolytic plasma. In a similar experiment using concentrated (140) hemolytic plasma from the blood collected on termination of extracorporeal circulation, agglutination of platelets was also confirmed. This indicated that ADP released in hemolysis agglutinates platelets and eventually causes iutravascular coagulation.
