Abstract
Perfluorochemical (PFC) emulsion has developed as artificial blood, but it is limited to a maximum of 1500ml in regard to the infusion volume because of poor excretion of PFC from body. However, if it is possible to retriev PFC once mixed with whole blood, this may increase the clinical applicability of PFC emulsion. We previously reported that centrifugation is a useful method of retrieving PFC from whole blood. Then this time, we tried normothermic Whole Body Rinse-out, almost same as Total Body Washout in efficiency, retrieving PFC and transfusing donor's fresh blood after replacement of blood with PFC emulsion. As results of the experiments using three dogs containing one jaundiced dog by ligation of common hepatic duct, bilirubin clearance rate was 90.5% and the concentration of PFC in blood reduced to 1/2-1/3 of that of maximum level.
