Abstract
In chronic hemodialysis patients, lymphoblastogenesis induced by PHA or Con A was studied in order to relate lymphocyte function to autologous plasma or human AB serum in different culture periods. Lymphocytes were collected obtained before dialysis, and that obtained at the arterial and the venous sides of the dialysis unit 15 minutes after the commercement of dialysis and at the end of dialysis. The highest response was seen both in lymphocytes from pre-dialysis blood and 15 minutes after commercement in three day cultures. The mitogen responsiveness of lymphocytes from blood obtained at the end of dialysis showed a tendency to decline with in days of culture. Addition of AB serum resulted significantly higher and more specific reactions to lymphocytes from blood obtained 15 minutes after dialysis commercement. Moreover lymphocytes from Cuprophan and PMMA membranes revealed differences in transformation capacity.
Autologous plasma as well as homologous serum showed specific response to lymphocytes from blood obtained 15 minutes after commercement of dialysis. Different ability was found between the arterial and the venous sides. Recognition of autologous plasma and homologous serum by lymphocytes from hemodialysis patients seemed fairly distinct.
