Modulation of bio-responce caused by blood perfused artificial organ: Adsorption of anphylatoxins by activated charcoal and inhibition of complement activation by using nafamstat mesilate (FUT)

Abstract

Previous studies have indicated that large amounts of anaphylatoxins are produced in blood perfused artificial organs. These anaphylatoxins are reinfused to patients. The reduction of reinfusing anaphylatoxins was attempted by charcoal adsorption and complement inhibitor (FUT). Activated charcoal adsorbed anaphylatoxins in serum and blood. Charcoal hemoperfusion (HP) was combined to clinical hemodialysis (HD). The peak of C3a decreased from 2700ng/ml (HD) to 700ng/ml (HD with HP). Transient neutropenia was suppressed in some HD by charcoal hemoperfusion. FUT was used for inhibiting complement activation in clinical plasmapheresis. The concentration of anaphylatoxins was lower with FUT than with heparin. Neutropenia was not affected with 20-100mg/hr of FUT.