Abstract
Several kinds of filters have been utilized to remove leucocytes and microaggregates through blood transfusion. In order to apply these filters to leucocytapheresis (LP), we examined the adhesive and activating capacity for blood cell components and complement levels respectively. Among three kinds of commercially available filters, Sepacell R 500 (S) showed the highest removal capacity for leucocyte and the absence of complement activation. In ex vivo LP with dogs, S showed sufficient adhesive capacity for leucocyte and coagulation during LP could be completely prevented by the use of ACD or nafamostat mesilate as anticoagulants. These results indicate that LP with S can be applicable clinically, but further improvements in the adhesive selectivity may be desirable.
