Abstract
A new method of polymyxin B fixed to insoluble fiber (PMX-F) was developed as a biomaterial for selectively detoxifing endotoxin (ET). This new material (PMX-F) neutralized a sufficient amount of ET, moreover this newly invented fiber exhibited a satisfactory biocompatibility. We previously reported the efficacy of PMX-F for endotoxin shock canine recived purified endotoxin. Our interest is to evaluate the capacity of this material to save septic dog as well as ET shock dogs. In this paper, direct hemoperfusion (DHP) with PMX-F was carried out for Escherichia coli (ATCC 25922)-induced septic dog. Septicemia was produced in ten dogs by dripped infusion of live Escherichia coli, 1-5×109organisms per kilogram of body weight for 1 hour. For five of ten dogs, DHP with PRIX-F was performed for 2 hours. Others were received sham DHP with empty column. We found that PMX-F treatment prolonged or increased survival rate in septic dog. DHP with PMX-F would be an effective treatment for the ET shock or septicemia in the clinical patient.
