1990 Volume 19 Issue 3 Pages 1064-1068
Many efforts to make an ideal vascular prosthesis with long-term patency and durability are continued from various points of investigation. Recently, concerning a small-caliber vascular prosthesis, endothelial seeding has been paid attention to. In this article, various methods to make a hybrid vascular prosthesis were reviewed and discussed. Preclotting method is a classical technique of endothelial seeding, but has a problem of uncertain ratio in cell attachment. Rotation method was developed for making a prelined graft which was endothelialized in vitro. Perfusion method was purposed to achieve physiological lining of the seeded endothelial cells. However, these methods of in vitro seeding without preclotting blood necessitated some coating material on the luminal surface of the graft. Filtration method was proposed under the condition of high density culture of endothelial cells. By this new method, endothelial cells primarily attached each fiber and chronologically formed a confluent monolayer. Hybrid vascular prosthesis is believed to be a new trend of vascular graft as the alternative of autogenous artery or vein.
