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[in Japanese]
1990 Volume 19 Issue 3 Pages
995
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
JOURNAL
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F. IWAYA, T. IGARI, T. ABE, K. HAGIWARA, M. TANJI, N. SATOKAWA, M. WAT ...
1990 Volume 19 Issue 3 Pages
997-1001
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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At 36 institutes in Japan, sixtyone patients with severe heart failure were treated by ventricular assist device (VAD) made by Nippon Zeon Co. Ltd. Thirty-four (56%) had been successfully weaned from the VAD and long-term survival of one month or more was achieved in 13 patients (21%) including 7 patients who have been discharged from hospital. It is very important to use VAD without hesitation when it is judged to be helpful.
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K TOKUNAGA, Y KAWACHI
1990 Volume 19 Issue 3 Pages
1002-1006
Published: June 15, 1990
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Durability and anti-thrombogenicity of the prosthetic heart valve and results of prosthetic valve re-replacement were discussed. In the isolated aortic valve replacement, the incidence of thromboembolism was significantly higher in the mechanical valve group (2.5±0.2%/P-Y vs. 0.5±0.3%/P-Y) (p<0.01). Structural valve failure was significantly more common in the bioprostheses group (0.2±0.2%/P-Y vs. 1.2±0.5%/P-Y) (p<0.05). The freedom from medical treatment in the late postoperative state was significantly higher in the bioprosthetic valve group (2.9% vs. 37.7%)(p<0.0001). In the isolated mitral valve replacement, the incidence of thromboembolism and anticoagulant-related hemorrhage was higher in the mechanical valve group (4.1±0.9%/P-Y vs. 2.2±0.4%/P-Y). Structural valve failure was significantly more comnnon in the bioprostheses group (0.4±0.3%/P-Y vs. 2.0±0.4%/P-Y) (p<0.01). In the isolated tricuspid or pulmonary valve replacement, valve failure was more common in the mechanical valve group (7.3±3.6%/P-Y vs. 0.5±0.5%/P-Y)(p<0.01). The valve-related events were more common in the mechanical valve group (9.1±4.1%/P-Y vs. 1.9±1.0%/P-Y)(p<0.001). In the prosthetic valve r-ereplacement, there was significant difference in the actuarial survival curve between single and double valve replacement group (p<0.001). In the single valve re-replacement for primary tissue failure of bioprostheses, actuarial survival rate at 5 years was 96±4%. We conclude that the benefit of bioprostheses (antithrombogenicity) is put to practical use in the case of aortic valve or right-side heart valve replacement.
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K. NAKANO, S. WATANABE, M. KITAMURA, Y. YAGI, A. SHIIKAWA, K. HIRATA, ...
1990 Volume 19 Issue 3 Pages
1007-1010
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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From July 1978 to December 1988, St. Jude Medical valve prostheses were implanted in 1199 patients. The actuarial survival rate was 86.1% in AVR, 93.8% in DVR. Valve-related complications was as follows: Structural failure (0), Thrombosis (AVR 1, MVR 2), Thromboembolism (AVR 10 (0.78%/pt-yr), MVR 24 (1.08), DVR 4 (0.57), Antieoagulant-related hemorrhage (MVR 1), Prosthetic valve endocarditis (AVR 3, `MVR 1, DVR 2), Hemolysis (AVR 7, DVR 1). Reoperat ion was performed in 14 patients. Judging from our 11-year experience, the St. Jude Medical valve is the prosthesis of our choice.
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A TAKEUCHI, S SASAKI, M OHZEKI, Y NISHIMOTO
1990 Volume 19 Issue 3 Pages
1011-1015
Published: June 15, 1990
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Four hundred and thirty two patients, treated with implantable pacemaker, were divided into two groups according to pacing modes (non-physiologic and physiologic pacing), Non-physiologic pacing (VVI)had not only some demerits oar patient's daily life and/or low cardiac output caused by disregard of atrial contribution such as pacemaker syndrome, but also showed significant decrease of survival rate in SSS group through whole observation period. The most important difference between two pacing groups was incidence of thromboembolic episodes, which was clearly higher in non-physiologic pacing than in physiologic pacing on SSS group.
Reason of favorable effect of physiologic pacing on avoiding thromboembolic episodes was presumably its AV synchrony, which might dissolve thrombogenetic factors in left atrium such as intraatrial hemostasis or conversion from regular atrial contraction to atrial fibrillation, For some cases, on which DDD pacemaker could not increase heart rate on exercise because of severe sinus node dysfunction, combination of rate responsive mechanism and DDD type pacemaker (DDD) should be the most suitable pacing mode.
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Shigeo TANAKA, [in Japanese], [in Japanese], [in Japanese], [in Japane ...
1990 Volume 19 Issue 3 Pages
1016-1019
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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We implanted 650 pacemakers in our clinic. The best survival ratio was achieved by physiological pacing with DDD and AAIR pacemaker. Non-physiological pacing with VVI and VVIR pacing should shorter longivity. Analysiny the quality of life by mailed opinion survay, the patients with RR-PM should worse results than that of fixed rate pacemaker patients. There is no clinical experience with AICD in Japan except seven experimental implants including one our care. Our conclusion is that with the ideal pacemaker with AICD function, improved patient longivity and quality of life is achieved.
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[in Japanese], [in Japanese]
1990 Volume 19 Issue 3 Pages
1020
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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S. TERAMOTO
1990 Volume 19 Issue 3 Pages
1021-1024
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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The oxygenator has been developed as an apparatus for open heart surgery. It is classified as (1) gas-blood interface type and (2) gas-membrane-blood diffusion type. Now, actually the former is a bubble oxygenator (BO) and the latter is a membrane oxygenator (MO). Recently, MO has been used more than BO in hospitals. Microporous membrane or dense (silicone) membrane is used for MO. ECMO (extracorporeal membrane oxygenation) is develped as new application of MO. ECMO is one type of assisted circulation by means of MO and is applied for acute respiratory failure (in original use) and for acute cardiopulmonary failure (in wide use). Bridge use for heart transplantation is also done sometimes. It might be developed the implantable artificial lung for respiratory failure in the future.
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T. AGISHI
1990 Volume 19 Issue 3 Pages
1025-1027
Published: June 15, 1990
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Treatment of renal failure is most popularized in Japan. Japanese specificity is pointed out to be, firstly, a large number of patientswho undertake the therapy, secondly, a biased dependency upon hemodialysis therapy rather than kidney tranplantation, and thirdly, multiplicity in blood purification modalities utilized for aritifial kidney. However, a one-year survival rate of the maintenance hemodialysis patients has not been improved for last several years. Treatment of the complications which may specifically appear to the long-term hemodialysis patients can be performed not by functional improvement of aritificial kidney, but by development of supplementary medical and surgical techniques. According to the nation-wide registry, almost a half of the hemodialysis patients who died died of cardiac failure and diseases of the cerebral blood vessels. Taking consideration of special lipid metabolism in the long-term hemodialyis patients into account, investigation of pathophysiology and medical correction of abnormal lipid metabolism is anticipated to help the well-being of the hemodialysis patients.
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H HIRASAWA, T SUGAI, Y OHTAKE, S ODA, H SHIGA, K MATSUDA, S KITAMURA, ...
1990 Volume 19 Issue 3 Pages
1028-1032
Published: June 15, 1990
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Since liver transplantation has not yet been clinically available in Japan, ALS with various hemopurifications remains to be a mainstay of the treatment. The indication of ALS should be decided according to the hepatocellular function. The criteria to initiate ALS are 1) arterial ketone body ratio<0.7, and 2) hepaplastin test<30% or total bilirubin>5mg/dl, and 3) depressed consciousness level or osmolality gap>2mOsm/kg/H
2O. The combination of plasma exchange, plasma adsorption and continuous hemofiltration is effective and useful as ALS for AHF.
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[in Japanese], [in Japanese]
1990 Volume 19 Issue 3 Pages
1033
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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T. MATSUDA
1990 Volume 19 Issue 3 Pages
1034-1041
Published: June 15, 1990
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The in vivo and in vitro tissue regeneration of artificial grafts were discussed from a biomaterial point of view. The emphasis was placed on the interplay of homo-and hetero-cell communities. The self-regenerated extracellular matrices and secreted humoral factors (cell growth factors and angiogenesis factors) primarily determine the tissue remodeling and tissue neovascularization. The current biomaterial technology serves as matrices for cell adhesion and growth. Surface design concept of enhancing more, bioactive role in tissue engineering was discussed. Prototype models of artificial basement membranes and extracellular matrices were presented.
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-LIVER-
T. AKAIKE
1990 Volume 19 Issue 3 Pages
1042-1046
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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In order to replace the liver function, the development of the system where natural hepatocytes are immobilized in a matrix polymer might be considered as the best way. We designed lactose-carrying polystyrene derivatives (PVLA) as a substratum for hepatocyte from the viewpoint of modeling asialoglycoprotein. On PVLA-coated dish, the specific function as well as attachment of hepatocyte was successfully maintained. Moreover, with the addition of EGF, the aggregation of lots of hepatocytes was made, which has better functions and longevity.
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Hiroshi AMEMIYA, Hiroo IWATA
1990 Volume 19 Issue 3 Pages
1047-1050
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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Host-versus-artificial organ reactions are discussed in comparison with organ transplantation. Laws of organ transplantation state that grafts will be accepted if the recipient shares Major Histocompatibility Complex (MHC) with the graft donor. Although extensive studies have been done to find the key properties, which correspond to MHC, to determine the biocompatibility of artificial organs, no such property have been found Immunosuppressant therapy is applied in organ transplantation. No effective drug such as immunosuppresive drug, however, could be found in artificial organs. Thus, not only researches on the biocompatibility of biomaterials should be continued, but also new drugs which can control reactions caused by foreign bodies should be found and more effort should be exerted to develop the methods to use anti-thrombogenic and anti-inflamatory drugs. Recently hybrid artificial organs are studying in order to promote the acceptance of articial organs.
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[in Japanese], [in Japanese]
1990 Volume 19 Issue 3 Pages
1051
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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Tadahiro SASAJIMA, Yoshihiko KUBO, Masaski KOKUBO, Yuiti IZUMI, Masash ...
1990 Volume 19 Issue 3 Pages
1052-1055
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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Factors affecting anastomoic intimal hyperplasia (AIH) were analyzed, There were two types of AIH: (1) occured at the host artery for a length of 5mm segment from the suture line. This rare type of AIH is mainly caused by compliance mismatch. (2) occurred 2S a form of punnus hyperplasia due to undequate blood and hist compatibility of the vascular prosthesis, and were frequently observed in Biograft and e-PTFE. We conclude that development of a compound vascular prosthesis with basement membrene is necessary in order to prevent the AIH.
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M. SAKUMA, T. NISHIBE, K. YASUDA, T. TANABE, G. HAYASHI
1990 Volume 19 Issue 3 Pages
1056-1059
Published: June 15, 1990
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An approach to develo ement of the ideal vascular prostheses; evaluation of anti-thrombogenesityand elastic properties of a small cal fiver segmented polyurethane graft.: Despite improvements of the vascular prostheses, the need for small caliver vascular prostheses remains unfulfilled. To improve the patency of small cal fiver grafts, the imporance of compliance in vascular graft design and anti-thrombogenecity are emphasized. In this study, new type segmented polyurethane grafts 3mm in diameter, which have the same compliance as human arteries and strong anti-thrombogenesity, were implanted in mongrel dogs and anti-thrombqgenecity and elastic properties of the grafts were evaluated by
111In platelet scintigraphy and laser compliance measuring system respectively. Platelet scintigraphy showed the same anti-thromboensity in this graft as in EPTFE graft and the patency rate 3 months after implantation was 50 percent. Compliance of the grafts was maintained favorably after implamtation and there was no significant differenceof stiffness-parameter β between control group and implanted group. This new type graft seems to have a good patency to replace small diameter arteres in the coronary and peripheral circulations.
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H. IJIMA, E. M. SAITOH, T. MURAI, Y. SAKAKIBARA, T. TSUTSUI, T. MITSUI ...
1990 Volume 19 Issue 3 Pages
1060-1063
Published: June 15, 1990
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There are many problems in the cardiovascular surgical field about use of synthetic vascular grafts.
However, low porosity woven Dacron grafts are frequently used for aortic surgery, its fitness with vascular wall is worse and its anastomosis is more difficult than those of high porosity knitted Dacron graft because of the rigidity.
We are not satisfied that the late patency rate of small caliber synthetic grafts around 5 or 6mm of internal diameter does not reach the level of that of autosaphenous vein except that of Sauvage EXS knitted Dacron. Anastomotic intimal hyperplasia is one of the most important problems and is difficult to control.
Venous reconstructive surgery using EPTFE grafts is in an era of trial and error yet.
Several clinical problems about currently available synthetic vascular prosthesis are discussed.
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-A NEW TREND OF VASCULAR GRAFT
A. TAKAGI, M. SHIRAKAWA, S. SHINDO, A. OSHIMA, T. MIYATA, J. EGAMI, T. ...
1990 Volume 19 Issue 3 Pages
1064-1068
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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Many efforts to make an ideal vascular prosthesis with long-term patency and durability are continued from various points of investigation. Recently, concerning a small-caliber vascular prosthesis, endothelial seeding has been paid attention to. In this article, various methods to make a hybrid vascular prosthesis were reviewed and discussed. Preclotting method is a classical technique of endothelial seeding, but has a problem of uncertain ratio in cell attachment. Rotation method was developed for making a prelined graft which was endothelialized in vitro. Perfusion method was purposed to achieve physiological lining of the seeded endothelial cells. However, these methods of in vitro seeding without preclotting blood necessitated some coating material on the luminal surface of the graft. Filtration method was proposed under the condition of high density culture of endothelial cells. By this new method, endothelial cells primarily attached each fiber and chronologically formed a confluent monolayer. Hybrid vascular prosthesis is believed to be a new trend of vascular graft as the alternative of autogenous artery or vein.
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-HOW TO CHOOSE GRAFTS FOR ARTERIAL RECONSTRUCTION IN OUR DEPARTMENT-
H. UCHIDA, Y. USUI, S. TERAMOTO
1990 Volume 19 Issue 3 Pages
1069-1073
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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We have been using porous Dacron prostheses according to Wesolowski's gossamer concept. Woven Dacron prostheses with porosity below 300ml/cm
2/min at 120mmHg have been chosen for the thoracic aortic surgery and high porous weft-knit Dacron prostheses with porosity between 2000 and 3000ml for the abdominal aortic and the distal arterial surgery. Our choice of vascular prostheses has been influenced by recent experimental and clinical results. An albumin-autoclave method and fibrin-glue preclotting are used to make the conventional woven and knitted Dacron grafts impervious. External velour warp-knit Dacron prostheses are gradually used for the abdominal aortic and the iliac arterial surgery. Externally supported Dacron prostheses are going to be used for the femoropopliteal area.
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S SATOH, S NIU, T OKA, H KURUMATANI, K WATANABE, Y NOISHIKI
1990 Volume 19 Issue 3 Pages
1074-1077
Published: June 15, 1990
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We have found the failure of the artificial vascular grafts with a relative small caliber that have been used clinically since 1970s. There were lots of late occlusion cases. The cause of the occlusion have been viewed from various aspects, for example, the difference of compliance, the flow disturbance or the anastomosis techniques. We have insisted that the deficiency of cell affinity of graft materials was the most important cause. Autocollagen has the high affinity for cells of the host. Using autologous connective tissue, we attempted to develop a vascular prosthesis with a small caliber. Moreover, to get stable autologous connective tissue around the framework, we have used ultrafine polyester fibers (UFPF) that provide an excellent microenvironment for infiltration and proliferation of fibroblasts. We have developed a new method of inducing antithrombogenicity to the connective tissue tube applied to a small diameter vascular graft. In combination of UFPF and this new method, we have developed a new type of small diameter autologous connective tissue tube (ACTT). It is concluded that ACTT like graft with good cellar affinity will fill the growing demand for small caliber grafts in clinical use.
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-SYNTHESIS AND INTERACTION WITH PLATELETS-
E. OZEKI, T. MATSUDA, T. AKUTSU
1990 Volume 19 Issue 3 Pages
1078-1082
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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In extracorporeal circuration, platelet aggregation and adhesion to materials were mediated by adhesive proteins. RGD (Arg-Gly-Asp)-peptides which is the fragment of adhesive proteins inhibit the binding of activated platelets to adhesive proteins. Based on NMR and CD conformational analyses, spacial orientation of Arg and Asp residues was found to be a principal determinant for binding of peptides to platelet receptors. Rigid spacial orientation with high activity was achieved by enhancement of β-turn like intramolecular hydrogen bonding and cyclization of peptide skeleton. Oligopeptides obtained showed dose-dependent inhibition of platelet aggregation. These compounds will be applicable as a novel platelet-preserving agent in extracorporeal circuration.
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-CLINICAL STUDY AS THE TUBE FOR DRAINAGE AFTER CARDIAC SURGERY
Y. OGURA, H. NAKAJIMA, J. ANBE, M. OZEKI, T. MITSUISHI, R. SUZUKI, T. ...
1990 Volume 19 Issue 3 Pages
1083-1086
Published: June 15, 1990
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Antithrombogenicity of the tube coated by PEO (PEOtube) was compared with polyvinylchloride tube (PVCtube), and clinicaly evaluated as the tube for drainage after cardiac surgery. By the study of scanning electron micrograph, fibrin formation on the surface of the PEOtube was inhibited comparing with the PVCtube. Patency of the PEOtube was better than PVCtube significantly (p<0.01) . Therefore it is suggested that longer drainage is possible by the PEOtube.
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-ANALYSIS OF 8 PATIENTS-
M. NISHIMURA, H. MATSUDA, N. OHKUBO, R. MATSUWAKA, R. YOTA, Y. KAWASHI ...
1990 Volume 19 Issue 3 Pages
1087-1091
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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The antithrombogenicity of polyurethane-siliconpolymer complex, K III 2, was evaluated for bypass tubing in assisted circulation in 8 patients. The PVC tubes coated with K III 2 exhibi ted a significantly better antithrombogenic performance in scanning electron microscopic findings (SEM) compared with non-coated PVC tubes. Although its efficacy was apparant when bypass flow was sufficient (more than 1.8l/min) in adult patients, it was not so effective in children and patients with low bypass flow. The results showed that the K III 2 coated tube was effective in adult patients with sufficient bypass flow, but it was not quite effective in children or adults with low bypass flow in preventing thromboembolism during assisted circulation.
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Y. SUZUKI, M. KUSAKABE, M. IWAKI, H. AKIBA, K. KUSAKABE, S. SATO
1990 Volume 19 Issue 3 Pages
1092-1095
Published: June 15, 1990
Released on J-STAGE: December 02, 2011
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H
+, N
2+ and O
2+ ion implantations in silicone were performed at 150keV to improve antithrombogenicity. The antithrombogenicity was tested by the superior vena cava (SVC) indwelling method, using In-111-tropolone-platelets. The accumulation of platelets onto H
+ implanted specimen and that of O
2+ implanted specimen and SVC decreased. The formation of functional groups induced by ion implantation depended on the ion species employed. The decrease in accumulation of platelets was related to new functional groups.
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M. CHIBANA, T. HIGAMI, K. OGAWA, T. ASADA, N. MUKOHARA, M. NISHIWAKI, ...
1990 Volume 19 Issue 3 Pages
1096-1099
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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The usefullness of Cell Saver for autotransfusion of shed mediastinal blood following open heart surgery was investigated in 18 patients (MVR 8 cases, CABG 6 cases, AVR 2 cases, OMC+TAP 1 case and Bentall's op. 1 case). Stored banked blood of 487±73ml (mean±SE) per patient was saved by this method and in 15 patients no transfusion was required postoperatively. The free hemoglobin in drainaged blood revealed high concentration (649.1±54.4mg/dl) but it decreased significantly to 99.8±13.5mg/dl after using Cell Saver. Similarly, anmonia, total birilubin, K
+, LDH, CPK, GOT, GPT, BUN, creatinine, total protein, white blood cell count and platelet count in drainaged blood decreased significantly. Red blood cell count, hemoglobin and hematocrit increased but there was no statistical significance. No patient had positive blood cultures and there were no unfavorable effect on the patients by this method. Consequently, we concluded that the autotransfusion of postoperatively drainaged blood treated by the Cell Saver after open heart surgery is safe and usefull.
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Y. NAGATA, H. TSUCHIOKA, M. KOBAYASHI, H. NOGAKI, K. SHIOI, H. SAIGUSA ...
1990 Volume 19 Issue 3 Pages
1100-1103
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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especially after cadiopulmonary bypass and neutrarization of heparin, we have manufactured for trial an intraoperative autotransfusion system. The system is simple consisting of suction sube, cardiotomy reserver with filter. A clinical evaluation was made in 18 patients. The use of the autotranfusion system had favorable results, 1300ml of homologous blood were reduced satisfactory on the average. No complications were observed. The use of this system during open heart surgery i s seem to be effective to reduce the amount of homologous blood transfusion.
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[in Japanese]
1990 Volume 19 Issue 3 Pages
1104
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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-ADHESIVE. NESS TO THE SKIN TISSUE THROUGH CUFF
S. WATANABE, N. OKUMURA, T. NATSUME, O. IKE, T. NAKAMURA, Y. SHIMIZU, ...
1990 Volume 19 Issue 3 Pages
1105-1108
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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We prepared an external shunt cannula with collagen-immobilized polyethylene sponge cuff for the purpose of attaining adhesiveness to the skin tissue through the cuff. Both ends of silicone cannulas with and without the cuff were implanted into the rabbit dorsal subcutaneous tissue. The skin tissue grew over and strongly adhered to the cuff of the silicone cannula. Attachment of the cuff was quite effective on adhesiveness to the skin and prevention of tunnel infections. This cuff can be used for peritoneal catheters for CAPD.
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K. MABUCHI, K. IMACHI, H. NOZAWA, T. CHINZEI, Y. ABE, T. YONEZAWA, M. ...
1990 Volume 19 Issue 3 Pages
1109-1113
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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Distributions of plasma proteins adsorbed on, and platelets adhering to, 8 artificial heart blood pumps were analyzed by both the autoradiography method using a radioisotope-conjugated antibody and by the immuno-peroxidase stain method. The distribution of the adhered platelets was also investigated using a scanning electron microscope (SEM). It was found that 1) the amount of Ig-G adsorbed showed a significant increase around thrombi, with a different distribution to that of albumin; and 2) the distribution of adsorbed fibrinogen and adhered platelets corresponded almost exactly to the sites of thrombus formation.
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Hitoshi MIYAKE
1990 Volume 19 Issue 3 Pages
1114-1117
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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The new actuator, the artificial muscle developed under the idea of biomimetic, have been composed of series/parallel connected artificial sarcomeres, which are made of the Ti-Ni shape memory alloy. It has some advantages of longer stroke, noiseless, and better controllability than the ordinary SMA actuator. This paper describes about some applications of the SMA artificial muscle for manipulators. Firstly, a single joint manipulator with push/pull antagonistic mechanism was developed. Secondly, a double joint manipulator was also developed. Both the manipulators showed good performances. Furthermore, a gripper with SMA artificial muscle was developed.
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N TAKAI, O OTSUBO, Y SUZUKI, J KAMO
1990 Volume 19 Issue 3 Pages
1118-1121
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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High membrane permselectivity for a bodyfluid mixture is wellknown to correlate with low permability of the desired product through the membrane. Exception to this rule exist, however, and this suggests the possibility of improved membranes for a number of important applications. This paper suggests posible carbon fiber which may allow control over the magnitudes of permeabilities and selectivities.
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K. MUNEOKA, T. TSUJI, T. TOGAWA, H. AOKI
1990 Volume 19 Issue 3 Pages
1122-1125
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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In the reconstruction of trachea with conventional biomaterials, the development of granulation at the anastomosis area is most important to influence the result. As an interface to the trachea, we used a tube made of sintered hydroxylapatite in combining with destruction of mucous membrane using a YAG laser. YAG laser irradiation with a special lateral irradiation probe can selectively destroy mucous secreting cells of circular region of the tracheal membrane and render uniformly the inner membrane sterile. Hydroxylapatite tracheal tube was succesfully associated with pretreatment of irradiation in rabbit.
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O. IKE, Y. SHIMIZU, T. OKADA, N. OKUMURA, T. NATSUME, S. WATANABE, Y. ...
1990 Volume 19 Issue 3 Pages
1126-1129
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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An artificial trachea was fabricated from a bioabsorbable poly (L-lactic acid) mesh coated with collagen. Our aim was to use this artificial trachea to reconstruct the respiratory airway by self-regeneration without retaining the prosthesis at the replacement site, thus preventing infection, anastomotic leakage, displacement of the prosthesis and subsequent stenosis. We substituted the cervical trachea of rabbits with the prosthesis and periosteum, which was sutured around it after being stripped from the tibia of the same animal. The cartilage produced from the periosteum around the prosthesis was seen 1 week after the operation, but no ossification was observed there after because of infection at the replacement site, perhaps due to inadequacy of the mesh for use as an artificial trachea. The internal surface of the cervical trachea was well epithelized without ossification 2 weeks after window-shaped replacements by the periosteal graft. On the other hand, ring-shaped bone tissue was recognized around the cervical trachea 9 weeks after insertion of the periosteal graft suggesting possible use for the treatment of tracheo- and/or bronchomalacia.
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[in Japanese]
1990 Volume 19 Issue 3 Pages
1130
Published: June 15, 1990
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M. KOIDE, J. KONISHI, K. IKEGAMI
1990 Volume 19 Issue 3 Pages
1131-1134
Published: June 15, 1990
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We investigated the characteristics of heat treated collagen matrix under various conditions in the presence of water, and the biocompatibility of these collagen matrix implanted in rats and mice. As a result, when collagen matrix was treated with high temperature, mechanical strength and protease resistance of heat treated collagen matrix was inferior to those of non-treated collagen matrix, and the contractibility abruptly increaseed with the increase of treated temperature. In impalnted experiment in rats, non-treated collagen matrix was extremely low in the filtration of cells and was observed encapsulation, whereas heat treated collagen matrix were remarkable observed the infiltration of cells and were not observed encapsulation.
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K. OSAKI, M. YAMAZAKI, A. GOTO, J. SAWAMOTO
1990 Volume 19 Issue 3 Pages
1135-1138
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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PVC or silicone with synthetic surface of very high water content were implanted abdominally in mice for up to 6 months. Hydrophilic surface modification was attaind by the undercoating formed a reactive functional group and the covalently bonded hydrophilic coating which is a copolymer of methyl vinyl ether and maleic anhydride. As a result, the hydrophilic coating layer obstructed adhesion, foreign body reaction, thick capsule formation, and chronic peritonitis. This finding suggests that this hydrophilic synthetic surface contributed considerable biocompatibility.
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Y. KADOMA, Y. IMAI
1990 Volume 19 Issue 3 Pages
1139-1142
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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Cardiothane films were subcutaneously implanted in the back of rats for up to 2 years. The retrieved films showed discoloration and opacification, but any apparent fragmentation or damage was not observed. SEM analysis revealed that fine cracks appeared after 6 months and then developed gradually. In ATR-FTIR spectra, the relative intensity of bands at 1367 and 1103cm
-1 due to polyether groups decreased. The 1729cm
-1 band of non-hydrogen bonded urethane carbonyl diminished significantly compared to the band at 1698cm
-1 of hydrogen bonded one. The hydrolysis of non-hydrogen bonded urethane linkage occurred during implantation and surface polyether content decreased. However, no appreciable changes in the tensile strength or the elongation at failure took place, since the degradation was limited only to the surface layer.
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T. MATSUDA, K. INOUE, T. AKUTSU
1990 Volume 19 Issue 3 Pages
1143-1146
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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In this paper, a novel surface patterning technology of cultured cells was developed. Based on photoreactive chemistry coupled with UV-irradiation, patterning of hydrophilic or hydrophobic surface characteristics were easily manipulated by using a photomask with a fine pattern. The irradiated surfaces after casting of photoreactive hydrophilic polymer resulted in chemical fixation. No adhesion of endothelial cells on treated surface and adhesion/grouwth on non-treated surfaces successfully formed two-dimensional patterning of cultured cells.
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H. NOMOTO, A. MAEDA, S. TOBE, T. AKAIKE, [in Japanese]
1990 Volume 19 Issue 3 Pages
1147-1150
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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We had reported that lactosecarrying polystyrene derivatives (PVLA) are specifically recognized by the asialoglycoprotein receptors of hepatocyte and that serum components have on significant effect on the attachment of hepatocyte. In this study, we minutely examined the role of serum factors e. g. albumin and fibronectin on the recognition of hepatocyte to several substrata such as PVLA, PVMA, polystyrene and so on.
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-BY LATEX PROBE METHOD-
A. YAGAWA, A. MAEDA, H. NOMOTO, S. TOBE, T. AKAIKE, K. KOBAYASHI
1990 Volume 19 Issue 3 Pages
1151-1154
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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We synthesized lactose-carrying polystyrene derivatives (PVLA). We reported that PVLA polymers take part in specific interaction with hepatic asialoglycoprotein recepter of liver parenchymal cell. We labeled asialoglycoprotein recepters with PVLA coated polystyrene latices.
PVLA coated latices did not attach to hepatocyte surface when hepatocyte were incubated on the culture dish coated with PVLA. This phenomenon shows that asialoglycoprotein recepter gradually clustered on the contacting surface (basal area) between hepatocyte and PVLA coated dish.
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[in Japanese]
1990 Volume 19 Issue 3 Pages
1155
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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S. TOBE, Y. TAKEI, A. MAEDA, T. AKAIKE, K. KOBAYASHI
1990 Volume 19 Issue 3 Pages
1156-1160
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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We synthesized artificial biomaterial as asialoglycoprotein model which abbreviated as PVLA. And so we found interesting phenomenon that cultured hepatocytes on PVLA substratum started gradual movement, and then remarkably formed multilayer aggregations which had long-term survival. The formation of multilayer aggregations clearly depended on supplemental concentrations of growth factors such as EGF and on numbers of seeded hepatocytes. Cultured hepatocytes on PVLA substratum required Caion in order to form multilayer aggregations. The cells in the aggregation exhibited better maintenance of specific functions for a long period.
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S. MIZUNO, K. YANAGI, N. OHSHIMA
1990 Volume 19 Issue 3 Pages
1161-1164
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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To attain highly concentrated culture of hepatocytes is an essential requirement in developing an artificial liver support system or a bioreactor. This study was attempted to improve the culture conditions of the primary hepatocyte culture. We tested 9 kinds of modified substrate materials, which were selected as major components of extracellular matrices, substances affecting surface charges, and a typical hydrophilic polymer. It is sugested that with chondroitin sulfate a spheroid-like aggregation of hepatocytes is inducible. Morphological studies revealed that spheroids have inner structures similar to liver cells
in vivo. They also keep sufficient viability, ammonia metabolism and albumin synthesis.
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H. TAKABATAKE, N. KOIDE, K. SAKAGUCHI, M. KAWAGUCHI, T. TAKENAMI, R. O ...
1990 Volume 19 Issue 3 Pages
1165-1168
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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It has been reported that hepatocyte-spheroids (spheroid) formed on a substrate of Liver-derived proteoglycans would be a suitable bioreactor for the hybrid artificial liver because of the easy handling and their differentiated functions maintained. In the present study, we surveyed simple substrates equivalent to proteoglycans as to spheroid forming ability. Polystyrene dishes with a positively charged hydrophilic surface, Primaria, appeared to be the simplest material effective for the spheroid formation. Any polystyrene materials (with positively and negatively charged surfaces, and hydrophobic and hydrophilic surfaces) that had been coated with bovine serum albumin or 2-hydroxymethyl methacrylate were also effective. Various characteristics reported for the spheroids formed on proteoglycan substrate, as to high albumin producing ability, poor proliferating ability and differentiated morphology, were equivalent to those formed in Primaria dishes. Noticeable difference was that the average diameter of spheroids formed on proteoglycan substrate was slightly smaller than the other. Thus we concluded that polystyrene with positively charged hydrophilic surface was a suitable material for the production of hepatocyte-spheroid in a large scale.
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O. IKE, T. NATSUME, Y. SHIMIZU, T. OKADA, N. OKUMURA, S. WATANABE, Y. ...
1990 Volume 19 Issue 3 Pages
1169-1172
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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The aim of our artificial esophagus was to regenerate the neoesophageal epithelium and to construct the neoesophageal lumen, thus preventing infection, anastomotic leakage, displacement of the prosthesis and subsequent stenosis. For this purpose, our artificial esophagus had a bilayered structure consisting of a silicone tube coated with collagen. After substitution of the cervical esophagus of adult mongrel dogs with our artificial esophagus, the dogs were denied oral food and given only intravenous hyperalimentation. Three to four weeks after the operation, 5cm of the replaced site was well epithelized and the neoesophagus was completed. In this study, in an attempt to shorten the time of neoesophageal epithelization, we injected 10-day-cultured buccal mucosal cells into the collagen layer of the artificial esophagus at the time of substitution. The neoesophagus was regenerated 1 week after the seeding without any local complications. Neoesophageal epithelization was completed 2 weeks after the seeding, thus shortening the time of the neoesophageal construction. Ultrastructurally, rough endoplasmic reticulums were evident in the submucosal fibroblasts, producing a bundle of collagen fibers.
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M. BABA, H. AJIKI, M. NAKAMURA, Y. NAKAKURA, Y. KIKUCHI, M. YAMAGISHI, ...
1990 Volume 19 Issue 3 Pages
1173-1176
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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Rastelli operation were performed using valved conduits in 3 cases whose diagnosis were PA with VSD. These valved conduits were made of xenopericardum in our technique. An anastomosis of the conduit to the pulmonary artery was easy compared with a woven dacron graft and any complications were not occured during the operation. Post operative hemodynamic study was carried out in two cases except of one who had infective endcarditis. These conduits were functioning well inthree cases with the longest follow up at 18 months. It was seemed xenopericardial valved conduits were useful material for external conduit.
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T. MATSUDA, K. INUE, T. AKUTSU
1990 Volume 19 Issue 3 Pages
1177-1180
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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A novel surface modification technology using photoreactive phenyl azide was developed especially to provide biocompatibility of surfaces of fabricated devices. Hydrophilic poly (dimethyl acrylamide) copolymer having phenyl azide groups were synthesized and cast on polymeric films. UV-irradiation resulted in chemical fixation of hydrophilic polymer on surfaces. A marked reduced cell adhesion (platelets and endothelial cells) were achieved, indicating that the technology developed here may find a potential application of surface modification of fabricated devices.
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[in Japanese]
1990 Volume 19 Issue 3 Pages
1181-1182
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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Koukichi YUASA, Takeshi SHIMIZU, Hisaya SHIRAKAWA
1990 Volume 19 Issue 3 Pages
1183-1185
Published: June 15, 1990
Released on J-STAGE: October 07, 2011
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We employed Silicone's materials in closing the defects left after resection of the chest wall in 9-patients with invasive carcinoma of the lung. Replacement with silicone materials were extremely useful due to its desirable rigidity and easy manipulation in operation. Neither paradoxical movement of the chest nor transformation of the prostheses occuured postoperatively in all of 9-patients. No disturbances of respiration nor chest wall movementand and no infection were experienced in these materials. 4 cases died of metastasis of lung cancer. The survival period of 4 months to 49 months was observed in other patients.
Operative results of chest wall reconstruction were satisfactory in this series of study. It is considered that silicone's materials is suitable as prosthetic material in the repair of chest wall defects.
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