ANALYSIS OF RECEPTERS DYNAMICS ON THE SURFACE OF ATTACHED HEPATOCYTE

-BY LATEX PROBE METHOD-

Abstract

We synthesized lactose-carrying polystyrene derivatives (PVLA). We reported that PVLA polymers take part in specific interaction with hepatic asialoglycoprotein recepter of liver parenchymal cell. We labeled asialoglycoprotein recepters with PVLA coated polystyrene latices.
PVLA coated latices did not attach to hepatocyte surface when hepatocyte were incubated on the culture dish coated with PVLA. This phenomenon shows that asialoglycoprotein recepter gradually clustered on the contacting surface (basal area) between hepatocyte and PVLA coated dish.