FORMATION OF MULTILAYER AGGREGATION OF ADULT RAT HEPATOCYTES IN PRIMARY CULTURE ON SUBSTRATUM AS ASIALOGLYCOPROTEIN MODEL

Abstract

We synthesized artificial biomaterial as asialoglycoprotein model which abbreviated as PVLA. And so we found interesting phenomenon that cultured hepatocytes on PVLA substratum started gradual movement, and then remarkably formed multilayer aggregations which had long-term survival. The formation of multilayer aggregations clearly depended on supplemental concentrations of growth factors such as EGF and on numbers of seeded hepatocytes. Cultured hepatocytes on PVLA substratum required Caion in order to form multilayer aggregations. The cells in the aggregation exhibited better maintenance of specific functions for a long period.