CONTINUOUS MEASUREMENT OF UREA IN WHOLE BLOOD USING CELLULOSE GEL COUPLED WITH UREASE

Abstract

To measure urea concentration in circulating blood continuously, we use column as a reactor for immobilized urease and ammonia was determined with glass electrode. Our system consists of two ammonia electrodes and urease immobilized to 500μm diameter, spherical cellulose gel. The system gave highly significant correlation between the emf of the electrode and log of urea concentration between 0.1mM and 100mM. The effect of erythrocytes on the reading could be eliminated using the voltage difference between the two ammonia electrodes before and after the reactor.