1990 Volume 19 Issue 3 Pages 1369-1372
A simple method to introduce cell components of blood vessels into a fabric vascular prosthesis was developed. This method showed prompt healing of the graft after implantation in an animal study. Tissue fragments suspension was prepared using a piece of peripheral vein. Highly porous fabric vascular prosthesis was immerced into the suspension fluid. The tissue fragments were trapped from the outer surface of the prosthesis by suction from the inside. Preclotting procedure was adopted to immobilize the tissue fragments to the fabric of the prosthesis. The prostheses were implanted into the thoracic descending aortae of 15 dogs. There was no bleeding at the time of the implantation. Numerous endothelial cells proliferation was observed in the specimen for 5 days after implantation. The surface was covered with thin layer of fibrin. Full endothelialization was noticed in the specimens of 35 days. The graft wall was completely healed. This method was very simple and easy to prepare, but the efficacy to promote the healing of the neointima was excellent.
