肝細胞spheroidを用いる人工肝モジュール作成の試み

高畠 弘行; 小出 典男; 佐々木 俊輔; 松島 寛; 武南 達郎; 小野 良策; 坂口 孝作; 船津 和守; 辻 孝夫

doi:10.11392/jsao1972.20.139

Bio-artificial liver using encapsulated hepatocyte spheroids

H TAKABATAKE, N KOIDE, S SASAKI, M MATSUSHIMA, T TAKENAMI, R ONO, K SAKAGUCHI, K FUNATSU, T TSUJI

Author information

H TAKABATAKE
The First Department of Internal Medicine, OkayamaUniversity Medical School
N KOIDE
The First Department of Internal Medicine, OkayamaUniversity Medical School
S SASAKI
The First Department of Internal Medicine, OkayamaUniversity Medical School
M MATSUSHIMA
The First Department of Internal Medicine, OkayamaUniversity Medical School
T TAKENAMI
The First Department of Internal Medicine, OkayamaUniversity Medical School
R ONO
The First Department of Internal Medicine, OkayamaUniversity Medical School
K SAKAGUCHI
The First Department of Internal Medicine, OkayamaUniversity Medical School
K FUNATSU
Department of Chemical Engineering, Faculty of Eng., Kyushu University
T TSUJI
The First Department of Internal Medicine, OkayamaUniversity Medical School

Keywords: Spheroid, Primary culture, Encap-sulation, Bio-artificial liver

JOURNAL FREE ACCESS

1991 Volume 20 Issue 1 Pages 139-144

DOI https://doi.org/10.11392/jsao1972.20.139

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Abstract

Rat hepatocyte-spheroids encapsulated with 2.5% calcium-alginate gel were used for a bioreactor in a circulating spouted bed culture system. The encapsulated spheroids in this system retained morphological stability as well as albumin and urea nitrogen producing abilities for 48 hours. The encapsulated spheroids may provide a new bio-reactor in bio-artificial liver support, since they had advantages of easy handling, functional and morphological stabilities and ability of high-density culture in this system.

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