Abstract
To clarify the mechanism of the loss of sensor's activity during the implantation, the effects of the three steps of blood coagulation on sensor output were investigated. When a needle-type glucose sensor was dipped into thrombo-cyte-rich saline injected with epinephrine to induce thrombocyte aggregation, the sensor showed no significant change in its output. When a sensor was dipped into thrombocyte-rich plasma injected with CaCI to induce fibrin clotting, the sensor also showed no significant change in its output. However, after the sensor was implanted in the artificial skin when fibroblasts were grown up, the sensor showed a negligible response against glucose in culture medium. These data indicated that cell-aggregation around an implanted sensor might interfere its output and a sensor's membrane design to reduce the cell-aggregation around a sensor might extend its long-life.
