Abstract
Porcine islet-like cell clusters (ICCS) were transplanted into streptozotocine-induced diabetic rats (STZ-rats) and NOD-mice in the absence of immunosuppression, using a diffusion chamber in a Bioartificial endocrine pancreas (Bio-AEP). An implantable diffusion chamber for a Bio-AEP was constructed by placing ICCs in a gel-matrix in the center of a ring holder sandwiched between two polycarbonate membranes which were held in place by two other ring holders, or just sealed with a silicon O-ring. The membranes were used as a mechanical barrier between the endocrine pancreatic graft and the host's immuno system. Transplantation of Bio-AEPs with ICCs into NOD-mice and STZ-rat re-versed hyperglycemia for at least 7 to 10 weeks without immunosuppression.
