Abstract
The substrate dependency of neoendothelialization was evaluated using an in vitro culture model with bovine aortic endothelial cells. The materials used were glass coated with four types of segmented polyurethanes and three kinds of proteins. The quantitative analysis of cellular behaviors including adhesion, spread-ing, proliferation and migration (velocity and directionality) defined determinant factors for in vitro endothelialization as follows: 1) Substrates with poor adhesive, spreading and proliferative responses resulted in immature endothelialization regard-less of migratory response. 2) The substrate with profound cellular responses in the every process was found to be best-suited for enhancement of endothelialization. Thus, endothelialization is determined by concerted processes of cellular behaviors, each of which largely depends on type of substrate and kind of proteins. This concept may be a logical basis of the development of biomaterial for vascular system.
