Abstract
The tap water was highly purified by reverse osmosis system, and the bicarbonate reservoir was cleaned and disinfected every day. Then, the cleaned dialysis fluid was moreover purified through the double ultrafiltration(UF) filters with 6, 000 dalton cut off point. The finally obtained dialysis fluid could exceed the authorized critical standard for the infusion fluid of the clinical use. This ultrapure dialysis fluid did not clinically increase the plasma endotoxin(ET)(4.1±1.8pg/ml, 1.8±0.9pg/ml), interleukin-1β(IL-1β)(9.3±5.1pg/ml, 9.3±4.9pg/ml) and serum amyloid protein-A (SAA)(12.9±4.2μg/ml, 9.8±5.3μg/ml)after the hemodialysis using the polysulfone dialyzer. Furthermore, this ultrapure dialysis fluid was infused for the substitution fluid through the additional double UF filter as the on-line hemodiafilt-ration(on-line HDF). Our clinical on-line HDF trial did not also introduce the rise in ET(3.1±1.9pg/ml, 2.3±1.1pg/ml), IL-1β(11.0±6.7pg/ml, 8.3±15.6pg/ml) and SAA(10.8±4.8μg/ml, 7.9±4.3μg/ml). Our data showed that the ultimate purification of the dialysis fluid was achi-eved by the present methods. In conclusion, the ultrapure dialysis fluid was able to be in-fused safely in the on-line HDF.
