Abstract
The amount of dialysis membrane charge affects electrolyte transport through membrane. But its exact charge has not been measured directly. The authors tried to use fluorescence method which is used for biomembrane potential measurement in order to measure hollow-fiber dialysis membrane charge. An intensity of anionic fluorescent dye;1-anilinonaphthalene-8-solfonic acid(ANS);increases when it binds to charged parts of hydrophobic substances. Measurement of fluorescent intensity was made with two kinds of hollow-fiber dialysis membranes of regenerated cellulose;negative charged RC and positive charged Hemophan. Little fluorescence detected with RC indicates no ionic bond between ANS and negative charged membrane. In case of Hemophan, extremely intensive fluorescent was observed, but fluorescent intensity was decreasing with a decrease of membrane charge. A fluorescent intensity of ANS is considered to reflect hollow-fiber membrane charge. Moreover, fluorescent intensity corresponds to the quantity of bound ANS in dialysis membrane. This may also make it possible to analyze dialysis membrane structure.
