Studies on the Metabolism of Fluoroacetate in the Liver of a Rat

Part II. Isolation and Identification of Monofluoroacetone Bodies

Abstract

Previous reports show that fluoroacetate could be activated even in a mammalian liver. To give a further evidence for the activation of fluoroacetate in the tissue, a rat liver isperfused with saline containing sodium fluoroacetate, and from the perfusion fluid monofluoroacetoneis separated as the 2, 4-dinitrophenylhydrazone, which is identified with the hydrazoneof synthetic monofluoroacetone on the phenoxyethanol-impregnated paper chromatogram.