ISLET ALLOGRAFT IN DOG USING NEW BIOARTIFICIAL PANCREAS OF BLOOD PERFUSION TYPE

Abstract

The efficacy was evaluated the bioartificial pancreas as new artificial vessel type in canine allograft model. We developed the bioartificial pancreas enclosing 10% photo-cross linkable polyvinyl alcohol (PVA-AbQ) hydrogel mixed with 70, 000-190, 000 of purified canine islets to the wall of knitted artificial vessel. This bioartificial pancreas was tranaplanted to the abdominal aorta of 5 diabetic dogs totally removed pancreas. We administered exogenous insulin to keep normal insulin after grating (0-10U/day) than that before transplantation (8-16U/day), and no rejection reaction was histologically observed. Bioartificial pancreas of artificial vessel type with PVA-SBQ was newpromising strategy to prevent rejection in islet allotransplantation in contact with blood stream for Type I diabetic patients.