WASHING OUT METHOD OF 2ND FILTER ON CRYOFILTRATION

Abstract

A washing out procedure of the 2nd filter is required on cryofiltration when the filter is filled with the cryogel. We changed the washing out method for an improvement of albumin loss. The purpose of this study is to compare albumin loss and reduction efficacy of other proteins between the prior method (method A) and the improved method (method B) in 5 patients. Method A was only washing back with 500 ml of saline solution. Method B was that the plasma within the 2nd filter was forced out to patient with 200ml of saline solution and then the filter was washed back with 300ml. Both reduction volume and rate of serum protein was decreased in method B. Those of albumin were significantly reduced in method B in 3 patients, in spite of reduction of albumin supply. As washing out of cryogel became incomplete in method B, the frequency of washing out and the time of treatment increased. The washing out procedure is so complicated that we have now developed an automatic washing out system.