Abstract
A response current may be changed by fouling caused by protein or fibrobrast adsorption when a glucose sensor is implanted. By making the slope of glucose concentration in an enzyme layer flat, the influence of the fouling layer can be disregarded. In the present study, ferrocene binding with bovine serum albumin was used as a mediator of the glucose sensor. The proportionality between transient current after activation of glucose oxidase controlled by pulse potential and glucose concentration was examined. As a result, reaction right after the activation was not controlled by glucose diffusion, and transient current was dependent on glucose concentration. In conclusion, glucose concentration is determinable by measurement of transient current after the activation of glucose oxidase in the enzyme layer, and this method is applicable to long-term monitoring of glucose concentration.
