Abstract
In order to clarify the formation and function of the neural network, the electric activity of neurons was studied by simultaneous and multi-points measurements. Two kinds of MED were used and the cortical and hippocampal cell of rats were cultivated. Cortical cells were cultivated over 2 weeks on MED and the periodic action potentials were detected. Those potentials were retarded by the additions of 5mM Mg2+ and 50μM APV. Most cells showed the synchronized action potentials and the cycle of potentials were shortened with the proceed of cultivation. It was deduced that synapse binding was increased and extended rapidly in the period from the 4th day to the 9th day of cultivation. It was deduced that the neural network was formed at the same time in accordance to the synchronization.
