REGULATION OF CELLULAR GENE EXPRESSION BY ARTIFICIAL MATERIALS IMMOBILIZED WITH BIOSIGNAL MOLECULES.

Abstract

Differentiation and apotosis were regulated by immobilized nerve growth factor (NGF) and tumor necrosis factor (TNF), respectively. They were pattern-immobilized on a cell culture polystyrene plate by photolithographic technique. The pattern-immobilization was confirmed by immuno-fluorescence micriscopy. PC12 cells formed neurocites only on the NGF-immobilized regions. In addition, the neurocites direction was regulated by immobilized NGF. On the other hand, A673 cells were apotosized only on the TNF-immobilized regions.