Whether xenotransplantation of trachea is possible by means of the graft treated with protease or not is the question to be solved in this study.
Pig trachea was used for xenograft. The graft was treated with 75% alcohol for 3 days, 10% hydrogen peroxide for 3 days, protease for 7 days and 0.5% glutaraldehyde for 3 days successively. Thereafter, each graft was sunk into silicon solution in which brittle graft was reinforced again (Fig 1). For protease, 0.05% Papain, 0.05% Ficin, and Varidase including 10, 000units/ml Streptokinase and 2, 500units/ml Streptodornase was used severally. Following biological and immunological tests were measured in the extract of thus treated grafts. Normal untreated pig trachea was used as control. 1) Protein content measurement by Lowryo's method, 2) Polysaccarides measurements --Glucose by Anthrone's method, Glucosamine by Elson-Morgan's method, 3) Passive hemagglutnation activity by SRBC, and 4) Delayed type hypersensitivity reaction (DTH).
Protein content in the trachea treated with each protease was remarkablly lower than normal trachea (Tab. 1). Similarly, Glucose in the treated trachea was significantly lower than normal, and Glucosamine in the graft treated with Papain and Varidase was also lower than normal. Thus, the antigenicity in the pig trachea was suspected to be decreased significantly by means of protease treatments, especially by Papain and Varidase. Humoral antibody response of the treated graft was lower than normal both in IgM and IgG (Tab. 3). DTH reaction showed that cell mediated immunity of the graft treated with Papain is lower than normal (Tab. 4).
In conclusion, above described treatments decreased the antigenicity of the grafts. Furthermore, xenotransplantation of the trachea is possible immunologically.