Analysis Of Sera Of Ureamic Patients With Liquid Chromatography

Abstract

This report was written about the basic study of the removal efficiency of many dialyzers. Shimadzu LC-1 LC-2 systems and TSK-G2000W (LS-170) gel were used.
Reprotein method; 1.0ml of H. D. sera diluted with saline (1:1) was ultrafiltrated with CF-50A. It needed 10 minutes filtration in 1000G.
Mobile phase; Buffers, approximate pH 6.4, showed best separation of peaks. In addition, mol concentration frcm 0.075 to 0.085 was best in phosphate buffer.
Peak contents; The middle molecule substances will be in ‘a’ peaks (Fig. 5).